After the successful ncRNA 2021 conference, we invite you to join us in Basel, Switzerland. The RNA field is rapidly evolving, with non-coding RNAs leading the way. Advanced technologies allow for sequencing full-length single RNA molecules at single-cell and sub-cellular levels, driving new discoveries in molecular biology, physiology, and disease. The sequencing of numerous genomes will enhance efforts to map and catalog ncRNAs, while advancements in synthetic oligonucleotides and genomics offer opportunities for RNA manipulation and engineering.